KMID : 0861020200350040037
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Korea Journal of Herbology 2020 Volume.35 No. 4 p.37 ~ p.43
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Anti-oxidant and anti-inflammatory effect of Allium Hookeri water extracts in RAW 264.7 cells
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Lee Sang-Soo
Han Hyo-Sang Yoo Ja-Yeon Nam Myung-Soo Kim Kee-Kwang
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Abstract
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Objectives : Allium hookeri is a well-known traditional herbal remedy and its root used for treatment of inflammation and tumor. However, the mechanism of anti-inflammatory effect of Allium hookeri is still unknown. This study aims to examine the mechanism of anti-inflammatory effect of Allium hookeri on mouse macrophage cell line, RAW 264.7 cells.
Methods : Anti-oxidant effect of water extract of Allium hookeri (WEAH) was measured by 2,2'-azino-bis-3- ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. 3- (4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)- 2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed to determine the effect of WEAH on cell viability in RAW 264.7 cells. In addition, anti-inflammatory effect of WEAH was investigated in RAW 264.7 cells. Inflammation of RAW 264.7 cells induced by lipopolysarccharide (LPS) treatment and expression levels of inflammatory cytokine interleukin 1 ¥â (IL-1¥â) and interleukin 6 (IL-6) gene were analyzed using quantitative reverse transcription PCR (qRT-PCR) analysis. Furthermore, the phosphorylation of inhibitor of nuclear factor kappa B (I¥êB¥á) after LPS treatment with WEAH-treated RAW 264.7 cells was confirmed by immunoblot analysis.
Results : WEAH showed a strong anti-oxidant effect and no cytotoxicity to RAW 264.7 cells up to 2 §·/§¢concentration. The LPS-induced mRNA expression levels of IL-1¥â and IL-6 were decreased by WEAH treatment. Furthermore, the LPS-induced phosphorylation of I¥êB¥á is attenuated by WEAH treatment.
Conclusions : Through experimental demonstration of anti-oxidant and anti-inflammatory effects of WEAH, we suggest that Allium hookeri is a valuable material for prevention and treatment of various inflammatory diseases.
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KEYWORD
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Allium hookeri, RAW 264.7 cells, Anti-inflammation, Anti-oxidant effect
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